|
KMID : 0811720040080000183
|
|
Korean Journal of Physiology & Pharmacology
2004 Volume.8 No. 0 p.183 ~ p.0
|
|
|
Functional Recovery Following Transplantation of Human Mesenchymal Stem Cell in Traumatic Spinal Cord Injury Model of Rats
|
|
Lee Kyung-Hee
Kim Eun-Shin
Choi Ji-Soo
Yoon Do-Heum
Park Yong-Gou
Lee Bae-Hwan
|
|
|
Abstract
|
|
|
Transplantation of adult MSCs directly into adult rat brain reduces functional deficits associated with stroke and express neural phenotype and migrate when placed in damaged brain. Although the differentiation of MSCs has been well studied, little is known about the influence of MSCs on spinal neural cells. What influence MSCs have on spinal cord, including neural stem cells, is a basic question that should be addressed before introducing MSCs into practical use. The aim of the present study was to investigate whether the transplantation of human MSCs into the spinal cord after a contusion injury promotes functional outcome. Spinal cord injury (SCI) was induced using NYU impactor at T9 spinal segment. Human MSCs which were coated with BrdU were transplanted 1 week after SCI. The behavioral test using BBB scaling was performed every one week for 2 months. Somatosensory (SSEPs) and motor evoked potentials (MEPs) were recorded to determine functional recovery. The pathological changes were examined by using immunohistochemistry. Hindlimb performance was modestly improved in transplanted group. In transplanted group, the evoked potential latencies were significantly shorter than control group. The pathological changes were examined by using immunohistochemistry such as BrdU, hTau staining. The BrdU and hTau positive cells were located at the injury epicenter and adjacent sites. The data showed improvement in functional outcome in animals treated with human MSC transplantation compared to control animals. Scattered cells derived from human MSCs expressed neural protein markers. These results suggest that transplantation of human MSCs may have a therapeutic effect after spinal cord injury. Supported by a grant of the Korea Health 21 R&D Project (01-PJ1-PG3-21302-0011).
Source: Korean Journal of Physiology & Pharmacology.2004 Oct;8(Suppl I):S136-S136
|
|
|
KEYWORD
|
|
|
Spinal cord injury, Human mesenchymal stem cell, Transplantation, Behavioral test, Electrophysiology
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|